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A specimen of the Hormonal drawer Drawer C · Hormonal

Human Chorionic Gonadotropin (hCG)

FDA Approved

A glycoprotein hormone FDA-approved for ovulation induction, cryptorchidism, and hypogonadotropic hypogonadism. Functions as an LH receptor agonist with over 50 years of clinical use in reproductive medicine. Gold standard trigger for assisted reproductive technology.

hCG · Choriogonadotropin · Pregnyl · Novarel · Ovidrel · Choragon · Profasi · Choriomon

Research evidence
High

38 human studies

Preclinical
16%
Clinical
84%

Based on 45 cited sources

Evidence Score95/100
Well-evidenced
Research Depth97/100
Mechanism98/100
Plausibility96/100
Global Coverage97/100
Community Experience82/100
Effectiveness85/100

clinically demonstrated · high confidence

Demonstrated effect magnitude — not a recommendation or safety claim.

Research Depth 97: Cochrane meta-analyses of multiple RCTs anchor the base (1A=35) — GnRH-agonist-vs-hCG trigger review 25358904 (17 RCTs, n=1,847), rhCG-vs-uhCG review 27106604 (18 RCTs, n=2,952), and cryptorchidism meta-analysis 7673426 (33 studies, 3,282 boys); low-RoB RCTs plus high-confidence AMSTAR-2 reviews (1B=24), total human N >>1,000 (1C=15), direct population/route/outcome match for approved uses (1D=15), solid but human-dominated preclinical base (1E=8). Mechanism 98: LHCGR is a defined receptor with binding + functional + antagonist/genetic confirmation (2A=40), full hCG->LHCGR->Gas->cAMP->PKA->steroidogenesis/ovulation chain mapped (2B=30), in-vivo dose-response (Coviello 15713727 linear ITT across 125/250/500 IU; 2C=18), confirmed in mammalian/human in vivo (2D=10). Plausibility 96: full mechanism->surrogate->clinical chain each independently supported (ovulation->live birth; ITT maintenance->preserved spermatogenesis 23260550; 3A=38), fully coherent with reproductive endocrinology (3B=25), LH/rLH class analogy (3C=19), tightly scoped single-mechanism claim (3D=14). Global Coverage 97: reproduced across many independent groups and countries (Cochrane pools span Europe/Israel/Canada/Australia/US/Egypt; 4A=33, 4B=24), >100 studies and still growing (4C=25), multi-regulator approval FDA + EU (4D=15). Community Experience 82: broad multi-venue TRT-adjunct discussion but niche vs population-scale use (5A=26), >7-year track record (5B=30), broadly consistent themes (5C=16), manageable/isolated adverse signals capping 5D=10. Effectiveness basis clinical (high): gold-standard ovulation trigger with ~88-95% ovulation rates, live-birth superiority over GnRH-agonist trigger in fresh IVF (25358904), hard patient-relevant outcomes, standard of care within its primary indication.

Scored June 2026 How we rate →
Evidence Level
high
Not approved for human use by any regulatory agency
Limited human clinical trial data
Consult a healthcare provider before use
Not FDA Approved WADA Prohibited
Identity
SCALE · 1:1 N-TERMINUS C-TERMINUS 237 AA · ~36,700 Da (heterodimer)
Also Known As
hCG • Choriogonadotropin • Pregnyl • Novarel • Ovidrel • Choragon • Profasi • Choriomon
Class
Heterodimeric glycoprotein
Length
237 amino acids
Mol. weight
~36,700 Da (heterodimer)
Sequence
Alpha (92 aa) + Beta (145 aa) non-covalently linked subunits
Molecular Structure
A
P
D
V
Q
D
C
P
E
C
T
L
Q
E
N
P
F
F
S
Q
Hydrophobic
Polar
Positive
Negative

hCG is a well-characterized hormone with extensive human clinical evidence spanning over 50 years. Its mechanisms are firmly established through receptor studies, clinical trials, and decades of therapeutic use.

How It Works (Simplified)

hCG mimics luteinizing hormone (LH) but lasts much longer in the body, providing sustained receptor stimulation:

Ovulation Trigger

Binds LHCGR on ovarian follicles, triggering final oocyte maturation and predictable ovulation 36-40 hours post-injection.

Testosterone Production

Stimulates Leydig cells in testes to produce testosterone, maintaining intratesticular levels critical for spermatogenesis.

Luteal Support

Maintains corpus luteum function, ensuring progesterone production needed to support early pregnancy until placental takeover.

Extended Duration

Half-life of 24-36 hours (vs LH’s 20 minutes) due to extensive glycosylation and C-terminal extension, enabling single-dose efficacy.

Key Research: Youssef MA et al. Cochrane systematic review (2014) established hCG as gold standard ovulation trigger with higher live birth rates than GnRH agonist in fresh IVF cycles. PMID:25358904

Important Limitations

  • OHSS Risk: Primary safety concern; hCG’s sustained LHCGR stimulation can cause ovarian hyperstimulation syndrome in susceptible patients
  • Not for weight loss: FDA has warned against hCG use for weight loss (ineffective and potentially harmful)
  • Requires monitoring: Fertility applications require physician oversight and ultrasound monitoring
  • WADA prohibited: Banned in male athletes due to testosterone-stimulating effects
i. LHCGR/cAMP Pathway · Primary Signaling
hCGLHCGRGαs proteinAdenylyl cyclasecAMP ↑PKA activation
OvaryMeiosis resumption, cumulus expansion, ovulation
TestisStAR/P450c17 activation, testosterone synthesis
Corpus luteumProgesterone biosynthesis
Mechanism LH/hCG receptor (LHCGR) agonism triggering cAMP cascade in ovarian follicles
Established 17 direct studies
Benefit shown to induce predictable ovulation for assisted reproduction
Evidence Level
High
17 Human
5 Animal
8 In Vitro
Mechanism Leydig cell LHCGR activation stimulating testosterone biosynthesis via StAR and P450 enzymes
Established 8 direct studies
Benefit shown to maintain intratesticular testosterone during TRT
Evidence Level
High
5 Human
3 Animal
4 In Vitro
Mechanism Corpus luteum LHCGR stimulation maintaining progesterone secretion
Established 12 direct studies
Benefit shown to support early pregnancy through luteal phase
Evidence Level
High
12 Human
4 Animal
3 In Vitro
Mechanism LHCGR activation in undescended testes promoting testicular descent
Supported 6 direct studies
Benefit may treat cryptorchidism in prepubertal males
Evidence Level
Moderate
33 Human
2 Animal
Mechanism Confidence
Established
Supported
Emerging
Evidence Level
High
Moderate
Low
Very Low

Reported positives

  • Effective for maintaining testicular function during TRT
  • Well-established clinical use with decades of data
  • Fertility preservation widely confirmed
  • Improved mood and sense of wellbeing reported

Reported negatives

  • Estrogen elevation requiring management
  • Water retention and bloating
  • Injection frequency burdensome (multiple times per week)
  • Emotional sensitivity and mood swings reported

“Standard component of TRT protocols. Extensively discussed in r/testosterone and HRT communities.”

Self-reported, unverified accounts — not clinical evidence and no substitute for the cited research. Anecdotes are prone to selection bias and placebo effects.

Phase 01 1
Hours 0-24

Rapid absorption following subcutaneous injection, with serum hCG rising quickly. Peak serum levels (Tmax) reached around 16-24 hours depending on formulation (urinary vs recombinant).

PMID:35137345
Phase 02 2
Hours 36-40

Ovulation occurs approximately 36-40 hours post-trigger injection in fertility protocols. This predictable timing enables precise scheduling of egg retrieval or timed intercourse.

PMID:25358904
Phase 03 3
Days 1-7

Sustained LHCGR stimulation due to 24-36 hour half-life. In males, testosterone elevation persists for several days. Corpus luteum support maintained through this period.

PMID:15713727
Phase 04 4
Weeks 2-4

With continued administration in TRT protocols, intratesticular testosterone maintained at near-baseline levels. Spermatogenesis preserved with ongoing treatment.

PMID:23260550

Research-based observations

This timeline reflects observations from published clinical and preclinical studies. Individual responses may vary significantly. This is not a guarantee of effects or a dosing schedule. Consult qualified healthcare providers for personalized guidance.

Good Signs (6 indicators)
Pharmaceutical-grade product from licensed manufacturer (Pregnyl, Ovidrel, Novarel)
Clear solution after reconstitution (urinary-derived) or pre-filled syringe (recombinant)
Proper cold chain storage maintained (refrigerated 2-8C)
Valid prescription from licensed physician
Expiration date clearly visible and not passed
Tamper-evident packaging intact
Warning Signs (5 indicators)
Compounded hCG from compounding pharmacy (variable quality control)
Product stored at room temperature for extended periods
Reconstituted solution stored longer than manufacturer recommendations
Discount pricing significantly below pharmaceutical cost
Online source without prescription requirement
Bad Signs (6 indicators)
Cloudy or particulate matter visible in solution
Product labeled for 'weight loss' or 'homeopathic hCG'
No prescription required for purchase
Sublingual or oral hCG products (not bioavailable orally)
Unknown manufacturer or missing lot numbers
Product shipped without temperature control
Positive quality indicator
Requires evaluation
Potential quality issue

For Research Evaluation Only

These quality indicators are general guidelines based on typical peptide characteristics. Professional laboratory testing (HPLC, mass spectrometry) provides definitive quality verification. This checklist is for initial visual evaluation only.

Synergistic
Compatible
Caution
Avoid

Kisspeptin stimulates endogenous GnRH/LH release while hCG provides direct LHCGR agonism. Combined use studied for ovulation induction with potentially reduced OHSS risk. Clinical trials support sequential administration.

GnRH pulses restore HPG axis function while hCG provides direct gonadal stimulation. Used sequentially in hypogonadotropic hypogonadism treatment protocols.

Different axes of action (GH vs gonadal). No known interactions; commonly used together in hormone optimization protocols.

Non-overlapping mechanisms. BPC-157 focuses on tissue repair while hCG acts on gonadal function. No interaction data available.

Both affect reproductive/sexual function through different mechanisms. PT-141 acts on melanocortin receptors while hCG stimulates gonadal hormone production. Monitor for additive effects.

Both stimulate gonadal function. Clomiphene via SERM action increasing LH, hCG via direct LHCGR agonism. Combined use requires careful monitoring for overstimulation.

Research Note: Interaction data is based on published literature, mechanistic understanding, and theoretical considerations. Most peptide combinations lack direct clinical study. This information is for educational purposes only and does not constitute medical advice. Always consult qualified healthcare providers.

This dossier synthesizes available evidence from peer-reviewed literature, regulatory documents, and clinical trial registries. Evidence strength ratings follow a modified GRADE approach.

For complete methodology details, see our Methodology page.

Important Disclaimer

This dossier is for educational purposes only and does not constitute medical advice. Always consult a qualified healthcare provider before making health decisions.

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